On the DEAE, try lower ionic strength or slightly higher pH,
try DEAE-Sepharose instead of cellulose, to get it to stick
Try a cation exchange column like carboxymethyl- or phospho-
cellulose; at slightly acidic pH if necessary.
eab
Narayanan Ramasubbu wrote:
Hi all:
I am trying to purify a pr
Hi all:
I am trying to purify a protein that was expressed in a baculovirus
system. The protein elutes in the flow through in a DE52 column. Further
gel filtration gives an enriched protein but we see that some medium
components tag along (weight and activity data do not match). Another
mutant
