Re: [ccp4bb] AW: [ccp4bb] Negative electron density in the Fo-Fc map at the binding site

ulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von > Armando Albert > Gesendet: Freitag, 18. Juli 2014 18:04 > An: CCP4BB@JISCMAIL.AC.UK > Betreff: [ccp4bb] Negative electron density in the Fo-Fc map at the > binding site > > Dear all, > I am screening a small library

[ccp4bb] AW: [ccp4bb] Negative electron density in the Fo-Fc map at the binding site

[mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Armando Albert Gesendet: Freitag, 18. Juli 2014 18:04 An: CCP4BB@JISCMAIL.AC.UK Betreff: [ccp4bb] Negative electron density in the Fo-Fc map at the binding site Dear all, I am screening a small library of ligands against my protein crystals. Following a soaking

Re: [ccp4bb] Negative electron density in the Fo-Fc map at the binding site

Hi Armando, can be many reasons, a few for instance: - are you sure the ligand you modeled in is the one that is actually there? - is the positive density around or overlaps with ligand atoms you places? Then that would means ligand parameters are underrefined (need more refinement) or not corre

[ccp4bb] Negative electron density in the Fo-Fc map at the binding site

Dear all, I am screening a small library of ligands against my protein crystals. Following a soaking with different ligands, I collect datasets to 1.9A resolution and refine them against an empty model without any problem. What is the meaning of a rather large negative electron density in th