Re: [ccp4bb] NCS restraints in Refmac5

2022-10-19 Thread Keitaro Yamashita
Dear Massimo, This was a bug in Refmac and has been fixed in Refmac5.8.0385 which will be hopefully released with the next CCP4 update. However, local NCS restraints do not impose restraints on B-factors, so you do not have to worry about that. Best regards, Keitaro On Thu, 13 Oct 2022 at 14:05

[ccp4bb] NCS restraints in Refmac5

2022-10-13 Thread Degano Massimo
Dear all, I have crystal with a protein homotetramer in the asymmetric unit, and I suspect from the electron density that the average B-factor of one chain may be substantially different than the others. Thus I would like to see the effect, if any, of changing the B-factor restraints between t