y, October 6, 2009 9:04:51 AM
Subject: Re: [ccp4bb] Mounting needle-shaped crystals
Hi Jack,
you could try the following strategy:
a) use a larger loop than your needle, then before freezing carefully dip the
loop onto the glass slide to reduce the amount of liquid
b) if your crystal is larger th
t;
>> http://www.xtals.org/pdfs/needles.pdf
>>
>> Artem
>>
>> -Original Message-
>> From: Artem Evdokimov [mailto:ar...@xtals.org]
>> Sent: Monday, October 05, 2009 9:05 PM
>> To: 'Tanner, John J.'; 'CCP4BB@JISCMAIL.AC.UK'
>&
Dear Jack
I can certainly recommend using the litholoops from molecular dimensions
to mount the crystal you descibe. Most of the membrane crystal we start
out with come as needles, the advantages is as mention before that the
slight twist in the nylon loops isn't present. We do not have much
-
From: Artem Evdokimov [mailto:ar...@xtals.org]
Sent: Monday, October 05, 2009 9:05 PM
To: 'Tanner, John J.'; 'CCP4BB@JISCMAIL.AC.UK'
Subject: RE: [ccp4bb] Mounting needle-shaped crystals
Hello felow MO crystallographer,
For all it's worth, a while ago I've co
as if
the sand were stone"
Jorge Luis Borges
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Tanner, John J.
Sent: Monday, October 05, 2009 5:52 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Mounting needle-shaped crystals
Dear CCP4,
I'
quot;
Jorge Luis Borges
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Tanner, John J.
Sent: Monday, October 05, 2009 5:52 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Mounting needle-shaped crystals
Dear CCP4,
I'm looking for advice on
Dear CCP4,
I'm looking for advice on mounting thin needles for low temperature data
collection. Our needles are fairly long (100-200 microns) but only 20
microns or less thick. When I pick them up with Hampton loops (0.05-0.1 mm
size), the crystals tend to break as they are moved out of the drop
