NCS.
Good luck,
Pete
-Original Message-
From: CCP4 bulletin board on behalf of Anjali Mehta
Sent: Thu 3/6/2008 7:47 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Molecular replacement of a multidomain protein
Dear All,
I am working with a Bifunctional protein of molecular weight ~60 kD
EPMR (now Open-EPMR, http://www.epmr.info) is an excellent alternative
for finding difficult, high-dimensional MR solutions. Experiment with
various resolution limits. We solved an asymmetric unit with 3
difficult-to-place dimers of low sequence homology by gradually
increasing the high-resolut
I've had a very good experience with MrBump:
http://www.ccp4.ac.uk/MrBUMP/
Not only because of the program itself, which was able
to find an unexpected template for the problematic
chain (the first one was straightforward in Phaser),
but also because of great support from Martyn & Ron.
It's defin
Dear All,
I am working with a Bifunctional protein of molecular weight ~60 kDa.
I have a 3.3 angstrom native dataset. The matthews number show there are 6
molecules in the asymmetric unit.
The structures of the individual domains are already known from prokaryotes.
The sequence identity with the k
