MR on CCs is generally a pain. The problem is that sliding any CC along
its own supertwist (say by one heptad) will give you a solution that
lines up very well with the "right" model. There are a lot of solutions
of this type, so you basically have a multitude of models that are all
"okay" an
ation Laboratory,
Menlo Park, CA.
-Original Message-
From: CCP4 bulletin board on behalf of Thomas Edwards
Sent: Fri 3/28/2008 5:57 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] MolRep of coiled coils
Dear BB,
I am attempting molecular replacement with a 2.8A data set from crystals of a
coiled
My short experience with coiled-coil is that molecular replacement can
be difficult for "classical software" (due to the very anysotropic
shape of the protein).
In our case (a short parallel dimeric coiled-coil), molecular
replacement trials using AMoRe or MOLREP were unsuccessful. We solved
the st
Sorry - I should have added that, yes, there are 2 identical peptide chains
that should be parallel coiled-coil.
Any advice gratefully received.
Ed
-Original Message-
From: cockburn [mailto:[EMAIL PROTECTED]
Sent: Fri 3/28/2008 1:35 PM
To: Thomas Edwards
Subject: Re: [ccp4bb] MolRep of
Dear BB,
I am attempting molecular replacement with a 2.8A data set from crystals of a
coiled coil of about 150 residues.
Probably p21212 but maybe p2221.
So far, Phaser, MolRep, Amore, Mr Bump, have not provided a good solution as
judged by Z-scores, CCs, Rfactors, and whether there is any den
