DLadakis,
The seed stock solution should be your crystallization solution with a
slight (5% maybe) increase in the precipitant. In my experience, I have not
needed additional protein to stabilize the nano-crystals, but as with all
things, every protein is different.
The idea is the crystals should
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Dear DLadakis,
you are probably thinking of using a robot for seeding. For manual
seeding you would overcome the problem of a stock solution by using a
cat whisker for seeding. Manual seeding also has the advantage of
getting a much better feeling for
Dear all
I would like your advice on preparing a seed stock for microseeding.
Should the seed stabilizing stock soltion include soluble protein as well. If
it doesn't will the seeds eventually disolve leading to an unsuccesfull screen?
Also how is the seedstock supposed to look under the microscop
