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> From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of mesters
> Sent: 28 September 2010 07:26
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] Micromatrix seeding
iner,
Roberto
Sent: Monday, September 20, 2010 10:21 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Micromatrix seeding using the mosquito
Dear Maria
In our hands Mosquito MMS worked by adding 2x protein solution + 1x seed
solution + 1x reservoir. The reason for doing this was two-fold:
I) r
Dear Maria
In our hands Mosquito MMS worked by adding 2x protein solution + 1x seed
solution + 1x reservoir. The reason for doing this was two-fold:
I) ratio protein/reservoir is similar to the condition that generated the seed
stock
II) a deliberate bias toward the initial condition is given
Dear all,
Anyone who have had success using the mosquito for micromatrix seeding?
Which way is the optimal way of adding seed solution to sitting drops
on an MRC plate?
Is it better to add 100 nl protein + 10 nl seed solution + 100 nl
reservoir solution or
to add concentrated seed solution
