Artem Evdokimov wrote:
(although Pt(IV) compounds seem to be fairly interested in -S-S- in my
experience).
I've certainly seen PIP interact (usefully for phasing) with -S-S- and
-S- (Met).
Cheers,
Charlie
--
Charlie Bond
Professorial Fellow
University of Western Australia
School of Biomedical,
Behalf Of Wataru
Kagawa
Sent: Tuesday, May 12, 2009 10:18 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Mercaptoethanol
Hi.
We are trying to prepare heavy atom derivatives of a protein in which
a surface residue was mutated to cysteine. Mass spectrometry of the
purified cysteine mutant
Hi Bill:
Thanks for the prompt reply. I will try purifying the protein without
mercaptoethanol.
Wataru
On 2009/05/13, at 11:42, William G. Scott wrote:
HI Wataru:
I think it makes an R-S-S-R' bond, so the disulfide will prevent the
heavy atom from binding, unless exchange is rapid.
I
Hi.
We are trying to prepare heavy atom derivatives of a protein in which
a surface residue was mutated to cysteine. Mass spectrometry of the
purified cysteine mutant showed an additional peak with a molecular
mass that correspond to a mercaptoethanol-bound cysteine mutant. I am
wondering
