Re: [ccp4bb] Membrane Protein expression and purification

2013-07-12 Thread krish
k you can be successful. >> >> Best of luck >> -Tim Craig >> Emerald Bio >> >> -- >> Date: Fri, 12 Jul 2013 00:29:28 -0400 >> From: krishna@gmail.com >> Subject: [ccp4bb] Membrane Protein expression and purification >

Re: [ccp4bb] Membrane Protein expression and purification

2013-07-12 Thread krish
a daunting task, but with enough hard > work you can be successful. > > Best of luck > -Tim Craig > Emerald Bio > > -- > Date: Fri, 12 Jul 2013 00:29:28 -0400 > From: krishna@gmail.com > Subject: [ccp4bb] Membrane Protein expression and purific

Re: [ccp4bb] Membrane Protein expression and purification

2013-07-12 Thread John Lee
ri, 12 Jul 2013 00:29:28 -0400 > From: krishna@gmail.com > Subject: [ccp4bb] Membrane Protein expression and purification > To: CCP4BB@JISCMAIL.AC.UK > > > Dear All, > > First of all sorry for bringing the non-ccp4 post. As our CCP4 community > is filled with expert

Re: [ccp4bb] Membrane Protein expression and purification

2013-07-12 Thread Timothy Craig
amount of detergents than you probably have thus far. Expressing membrane proteins can be a daunting task, but with enough hard work you can be successful. Best of luck-Tim CraigEmerald Bio Date: Fri, 12 Jul 2013 00:29:28 -0400 From: krishna@gmail.com Subject: [ccp4bb] Membrane Protein

[ccp4bb] Membrane Protein expression and purification

2013-07-11 Thread krish
Dear All, First of all sorry for bringing the non-ccp4 post. As our CCP4 community is filled with experts in various fields of structural biology: I'd like to get some help/suggestions from the membrane proteins expert community. I'm trying to express my membrane protein in Pichia pastoris (SMD11