Hello Rhys,
I suggest using an assay such as that described in Anal. Biochem.
336:117 to measure the amount detergent in your concentrated samples.
I found this assay accurate and easy to use with DDM. It should work
with b-OG too.
Good luck,
Ho
Ho Leung Ng
University of Hawaii at Manoa
As
e.
>
>
> Rhys
>
> ____________
> From: Jim Fairman [fairman@gmail.com]
> Sent: 09 May 2013 20:58
> To: RHYS GRINTER
> Cc: ccp4bb
> Subject: Re: [ccp4bb] Membrane Protein Optimisation
>
> With information you've provided I have mul
lded crystals seemed biased for the formation of the gels.
Thanks again for the help, I feel I might have a long raod to optimisation
ahead of me.
Rhys
From: Jim Fairman [fairman@gmail.com]
Sent: 09 May 2013 20:58
To: RHYS GRINTER
Cc: ccp4bb
Sub
With information you've provided I have multiple suggestions for you, some
of which you may have already tried:
1. OMPs can be fairly particular about which detergents they will
crystallize in. Try exchanging the protein into a different
detergent/detergent mixture and then set up new trays in
Hi Rhys,
I suspect that what you call a gel might be phase separation (correct me if
this is wrong) like an oily phase enriched in protein and detergent. you
may have too much detergent in your drop.
may I ask what detergent you are using (low or high CMC) and at what
concentration of detergent do
Hi All,
A quick question if you've ever worked on membrane proteins, I'm trying to
optimize crystals for bacterial integral outer membrane protein I'm working on.
I'm getting some fairly modest rod like crystals in a 0.1M Tris pH 7.5, 30%
PEG 600, 0.03M MgCl2 condition. However I get lots and
