CP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Matthews coefficient
Dear Xinghua,
Of course, you can have 2 molecules in the asu. However, if things don't
it is worth to double-check your space group or check for twinning.
Cheers
christian
xinghua qin wrote:
> hi CCPeers
> The Matthews
Dear Xinghua,
Of course, you can have 2 molecules in the asu. However, if things don't
it is worth to double-check your space group or check for twinning.
Cheers
christian
xinghua qin wrote:
> hi CCPeers
> The Matthews coefficient of my protein is 3 calculated with
> matthews-cell content ana
On Fri, Jun 25, 2010 at 12:41 AM, Francois Berenger wrote:
> When you say "After molecular replacement you should check the result
> with the model building program of your choice and correct as many
> errors as possible before running a refinement program."
>
> Can this step be automated in some
Vellieux Frederic wrote:
Hi,
No such thing as a stupid question. If the resolution is sufficient,
arp_warp does a good job. But it is a reconstruction and REFINEMENT
program. But checking the proper packing (that there are indeed contacts
in the 3 dimensions of space to form the crystal): tak
Hi,
No such thing as a stupid question. If the resolution is sufficient,
arp_warp does a good job. But it is a reconstruction and REFINEMENT
program. But checking the proper packing (that there are indeed contacts
in the 3 dimensions of space to form the crystal): takes only 10 seconds
using
Hello,
I am not a crystallographer, so I will ask
a maybe stupid question.
When you say "After molecular replacement you should check the result
with the model building program of your choice and correct as many
errors as possible before running a refinement program."
Can this step be automated
Dear Xinghua,
the solvent content provided by yhe Matthews-program is certainly correct.
Whether or not it applies to you protein is a different issue. With only 2
molecules in the asymmetric unit the solvent content rises to 65%, and this is
stll perfectly fine for proteins. So carry out molecula
asymmetric unit in my crystal.
Fred.
> Message du 25/06/10 07:54
> De : "xinghua qin"
> A : CCP4BB@JISCMAIL.AC.UK
> Copie à :
> Objet : Re: [ccp4bb] Matthews coefficient
>
> Hi everyone:
> Thanks for all the responses.
> The Matthaws-cell content analysis prog
Hi everyone:
Thanks for all the responses.
The Matthaws-cell content analysis programe in CCP4 package gives the
results: 47% solvent content and 3 molecules in asu with 87% confidence.
the space group is P3121. how to carry out self rotation function? can
phaser do that work? If there a
hi CCPeers
The Matthews coefficient of my protein is 3 calculated with matthews-cell
content analysis CCP4 programe with 87% confidence , but when doing the
refinement the third molecular couldn't get into the unit cell because of
too many clashes.Deletion of the clashed AA did not work well, T
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