Dear all,
I would like to thank everyone for being so kind and sparing their time to
respond. Just to summarise from all the responses that I got that may help
someone else:
MBP on its own is monomeric and tends to 'magically' help pretty much every
protein you throw at it to become soluble. In m
not the views of The University of Queensland.
From: Gang Dong mailto:gang.d...@univie.ac.at>>
Reply-To: Gang Dong mailto:gang.d...@univie.ac.at>>
Date: Wed, 24 Jul 2013 18:53:27 +0200
To: mailto:CCP4BB@JISCMAIL.AC.UK>>
Subject: Re: [ccp4bb] Maltose binding protein as a tag
De
ent: Wednesday, July 24, 2013 3:07 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Maltose binding protein as a tag
Dear all,
I would like to ask if someone has experience with maltose binding protein
(MBP) as a tag.
My protein fused to MBP seems to form oligomers that I find difficult to
prev
Dear all,
I would like to ask if someone has experience with maltose binding protein
(MBP) as a tag.
My protein fused to MBP seems to form oligomers that I find difficult to
prevent and I was wondering if mbp behaves similar to gst and may also
be prone to dimerisation/oligomerisation
Many thanks
