1) How can I make a mask as mentioned above? Am I doing it the
wrong way?
You can run NCSMASK with your first placed domain and generate a
suitable mask file for DM.
2) Is there any other method to improve the density of the
missing structure? I tried several MR probes of the sec
Hi
Have you checked for twinning?
J
TC Hu <[EMAIL PROTECTED]> wrote:>
> Dear all,
>
>
>
> I am working on a structure which is composed of two domains. The X-ray
> data
> is quite good (P21, 2.2A, Rmerge=0.08, 93% completeness), there is one
> molecule (277 residues) per ASU. The structure h
The R and Rfree after refining domain 1 are 39% and 44%. The
density is good for the existing model but disconnected in the
missing part of the structure in which I can hardly place any residue.
2) Is there any other method to improve the density of the
missing structure? I tried sever
Dear all,
I am working on a structure which is composed of two domains. The X-ray data
is quite good (P21, 2.2A, Rmerge=0.08, 93% completeness), there is one
molecule (277 residues) per ASU. The structure has four homologous
structures with sequence identities ranging from 35%-40%. The four
stru
