Re: [ccp4bb] Low Phaser RFZ

Dear Eric, I've just clarified the section in our documentation on how to tell if Phaser has solved your structure: http://www.phaser.cimr.cam.ac.uk/index.php/Molecular_Replacement#Has_Phaser_Solved_It.3F Briefly, the RFZ score is not that diagnostic of success or failure, particularly for high

Re: [ccp4bb] Low Phaser RFZ

Hi Eric For me it is perfectly normal Please check Phaser documentation : http://www.phaser.cimr.cam.ac.uk/index.php/Molecular_Replacement#Has_Phaser_Solved_It.3F All the best Carlos On 05/19/2015 02:36 AM, Eric Karg wrote: Hi all, Running Phaser using the apo protein as search model on a ~

Re: [ccp4bb] Low Phaser RFZ

Hi Eric, What does your map look like? Do you see features that don't come from the search model? That's the key. That said, with a TFZ of above 10, I'd be rather positive about my prospects. Andreas On Tue, May 19, 2015 at 2:36 AM, Eric Karg < 052044071b36-dmarc-requ...@jiscmail.ac.uk

[ccp4bb] Low Phaser RFZ

Hi all, Running Phaser using the apo protein as search model on a ~2.5 A dataset of a protein-DNA complex, I get a single solution but with low RFZ. The map looks reasonable but I was wondering why the RFZ is so low. Would this solution be acceptable? SOLU SET RFZ=3.2 TFZ=8.4 PAK=0 LLG=66