Thanks all for information and tips.
With the help, I did some quick'n dirty analysis
Total PDB (2011-01-11)
70303
Method Xray
61092
H
(A tad delayed)
Actually, typically we set up crystal trials for both cleaved and
uncleaved protein right away at the same time (if there's enough protein
and the cleavage works).
Cheers
phx
On 07/01/2011 15:10, Bosch, Juergen wrote:
I vaguely remember a paper by Aled Edwards, where they did
Rose
RCSB PDB
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Snijder,
Arjan
Sent: Friday, January 07, 2011 6:41 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Looking for PDB His-tag statistics
Dear all,
Best wishes for the new year!
I am looking for
I vaguely remember a paper by Aled Edwards, where they did the statistics for
their targets at SGC. As far as I recall they engineer with cleavable tag
purify and cyrstallize it first with tag and only if they don't get crystals or
a structure they cleave off the tag and rerun the screens. I thi
Dear all,
Best wishes for the new year!
I am looking for some statistics about the fraction of protein structures in
the pdb that are actually crystallized with a His tag, and from those
crystallized with a His tag whether these construct were engineered with a
cleavable tag or not. Has an
