than Na or Li salts.
>
> Artem
>
> -Original Message-
> From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
> Subscribe Ccp4Bb Kn L
> Sent: Wednesday, December 17, 2008 9:26 PM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: [ccp4bb] LDAO & SDS-PA
cribe Ccp4Bb Kn L
Sent: Wednesday, December 17, 2008 9:26 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] LDAO & SDS-PAGE
Hello everyone...
I have a protein solubilised in 1% LDAO, 50 mM Phosphate pH 7.5, 150
mM
NaCl. When I mix it with laemmli buffer and heat at 95C, 3 mins for
SDS-PAGE, it a
esday, December 17, 2008 9:26 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] LDAO & SDS-PAGE
Hello everyone...
I have a protein solubilised in 1% LDAO, 50 mM Phosphate pH 7.5, 150 mM
NaCl. When I mix it with laemmli buffer and heat at 95C, 3 mins for
SDS-PAGE, it aggregates (as in when you mix GuHCl
Hello everyone...
I have a protein solubilised in 1% LDAO, 50 mM Phosphate pH 7.5, 150 mM
NaCl. When I mix it with laemmli buffer and heat at 95C, 3 mins for
SDS-PAGE, it aggregates (as in when you mix GuHCl with SDS) and can't be
loaded onto the gel.
Does anyone know why it happens? Thanks a lot
