[ccp4bb] Jeffamine M-600 pHing recipe

2009-04-23 Thread Jacob Keller
2009 8:41 PM Subject: RE: [ccp4bb] Jeffamine M-600 pHing recipe Hello Jacob: It takes about 132 microliters of concentrated HCl per every gram of Jeffamine M-600 to get the pH to 7.5 at 25 degrees Celsius. From there, one can titrate to pH 7 using dilute or 1.0 M HCl to the desired measured pH.

Re: [ccp4bb] Jeffamine M-600 pHing recipe

2009-04-23 Thread Annie Hassell
kline 5 Moore Drive RTP, NC 27709 919/483-3228 919/483-0368 (FAX) annie.m.hass...@gsk.com "Jacob Keller" Sent by: "CCP4 bulletin board" 22-Apr-2009 20:29 Please respond to "Jacob Keller" To CCP4BB@JISCMAIL.AC.UK cc Subject [ccp4bb] Jeffamine M-600 pHing rec

[ccp4bb] Jeffamine M-600 pHing recipe

2009-04-22 Thread Jacob Keller
Hello All, and sorry for the stream of non-software-related posts, but does anybody have a recipe for how much 1M or concentrated HCl is needed to pH Jeffamine M-600 down to ~7? It seems that once the amines are protonated, there should be little buffering strength, so a rough recipe for pH 5-