Re: [ccp4bb] Incorrect chiral volumes error during real space refinement

2017-02-28 Thread Ries, Anne
Hi Arjen, thank you so much! Yes, I am working with coot, and you just solved a problem that bothered me for days! We found the same box also on the R/RC button on the toolbar. It’s called Refinement Weight. Best regards, Anne On 28 Feb 2017, at 17.36, arjen jakobi mailto:xtalst...@gmail.c

Re: [ccp4bb] Incorrect chiral volumes error during real space refinement

2017-02-28 Thread arjen jakobi
Dear Anne, You do not mention it, but from your description I infer that you are using Coot? EM potential maps differ in characteristic properties from crystallographic maps. The problem you describe likely comes from inappropriate weighting of the map term in the refinement target. You may need

[ccp4bb] Incorrect chiral volumes error during real space refinement

2017-02-28 Thread Ries, Anne
Hi, I am working with a cryo EM map at 4.2 A and would like to de novo build a model into the map. As our map is initially calculated in .mrc format, we converted it into .mtz format. I used a poly-Ala helix .pdb file as a model to begin with. I opened the .pdb of the helix and the .mtz map in