Re: [ccp4bb] Improving MR map contrast

2015-07-06 Thread Boaz Shaanan
ly 06, 2015 2:48 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] AW: [ccp4bb] Improving MR map contrast Dear Rhys,   what you are trying to do is at the edge of what is possible and you may, or may not succeed. It will also depend on the percentage solvent in your crystals. The more solvent, the b

[ccp4bb] AW: [ccp4bb] Improving MR map contrast

2015-07-06 Thread Herman . Schreuder
von Rhys Grinter Gesendet: Montag, 6. Juli 2015 13:08 An: CCP4BB@JISCMAIL.AC.UK Betreff: [ccp4bb] Improving MR map contrast Hi All, I recently obtained a molecular replacement solution for a 100kDa protein I'm working on, using an ensemble of low sequence identity models for around 1/4 of

[ccp4bb] Improving MR map contrast

2015-07-06 Thread Rhys Grinter
Hi All, I recently obtained a molecular replacement solution for a 100kDa protein I'm working on, using an ensemble of low sequence identity models for around 1/4 of the protein into 2.6A data. I got initial Tfz scores of around 8.2 (LLG of 100 or so), which I improved to 14 (LLG 350) which manual