otron will bless you.
Zhijie
From: Pradeep Mandal
Sent: Saturday, May 12, 2012 7:32 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Improvement of diffraction quality
Hi,
We are trying to crystallize (hanging drop vapor diffusion method, at 20degC) a
40kDa enzyme (conc. = 20mg/mL) using 0.1-
cp4bb] Improvement of diffraction quality
To: CCP4BB@JISCMAIL.AC.UK
Hi,
We are trying to crystallize (hanging drop vapor diffusion method, at 20degC) a
40kDa enzyme (conc. = 20mg/mL) using 0.1-0.2 M HEPES (pH = 8), 20-30% PEG 4K
and we obtain needle-like crystals in 2 days. These needle-like cry
Hi,
We are trying to crystallize (hanging drop vapor diffusion method, at
20degC) a 40kDa enzyme (conc. = 20mg/mL) using 0.1-0.2 M HEPES (pH = 8),
20-30% PEG 4K and we obtain needle-like crystals in 2 days. These
needle-like crystals diffract up to 4 Angs but decay in the X-ray beam in
spite of us
