cturing, Parkville)
Sent: Thursday, August 1, 2019 6:29 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Importance of temperature during initial crystallization
screening
Interesting topic,
Certainly the two papers suggested by Georg are relevant, and I fully agree
with the comments from Dani
Dear Sergei,
There is also this high temperature crystallisation study of lysozyme, up to 55
degrees C:-
https://febs.onlinelibrary.wiley.com/doi/abs/10.1016/0014-5793%2872%2980273-4
Best wishes,
John
Emeritus Professor John R Helliwell DSc
> On 1 Aug 2019, at 10:23, Sergei Strelkov wrote:
>
eath Campus, Cardiff, CF14 4XN
>>
>> email: rizkall...@cardiff.ac.uk <mailto:rizkall...@cardiff.ac.uk>
>> phone: +44 29 2074 2248
>>
>> http://www.cardiff.ac.uk/people/view/126690-rizkallah-pierre
>> <http://www.cardiff.ac.uk/people/view
ject: Re: [ccp4bb] Importance of temperature during initial crystallization
screening
Interesting topic,
Certainly the two papers suggested by Georg are relevant, and I fully agree
with the comments from Daniel that it is hard to predict the behaviour of any
given protein from a statistical ana
JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Importance of temperature during initial crystallization
screening
Interesting topic,
Certainly the two papers suggested by Georg are relevant, and I fully agree
with the comments from Daniel that it is hard to predict the behaviour of any
given protein from
Behalf Of Georg Mlynek
Sent: Friday, 2 August 2019 5:09 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Importance of temperature during initial crystallization
screening
Hi Sergei, this publication should be useful for you.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4756611/
Additionally it
@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Importance of temperature during initial crystallization
screening
Hi Sergei, this publication should be useful for you.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4756611/
Additionally it is proposed that when your protein is not so stable (lower Tm),
one should
Sergei,
That depends heavily on the protein, so I don't know if a statistical study
will accurately reflect the
importance of temperature. With some proteins, no matter how thoroughly
you sample chemical
space, temperature is critical, and a study would have to focus on such a
protein. The data
Hi Sergei, this publication should be useful for you.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4756611/
Additionally it is proposed that when your protein is not so stable
(lower Tm), one should incubate the screens at 4C.
http://scripts.iucr.org/cgi-bin/paper?S0907444911036225
Br, Georg
Hi Sergei
We did some data-mining on this way, way back, in 2004.
See the second section in this link
https://www.douglas.co.uk/PDB_data.htm
When you consider the *non-standard *temps - ie NOT 4C or 20C - it *looks*
as though the higher-end temps *may *work better. But of course it's hard
to
Dear all,
I wondered if someone could point me to a recent study on the importance of
temperature during initial search for crystallization conditions. It would be
interesting to see any real statistics on this subject.
We typically try to perform screening at at two temperatures, such as
du
11 matches
Mail list logo
