Le Vendredi 14 Mars 2014 13:37 CET, Anita P a écrit:
Anita,
If one of the partners is indeed more or less unfolded before interaction,
then you should see a negative DeltaS upon complex formation.
Practically, I would try first putting the unfolded protein in the cell and the
folded one in t
ilto:y...@cbse.uab.edu>
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Anita P
Sent: Friday, March 14, 2014 8:57 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] ITC with unfolded proteins
That is a very interesting question, which I would request the seniors
>
>> Reza Khayat, PhD
>> Assistant Professor
>> The City College of New York
>> Department of Chemistry, MR-1135
>> 160 Convent Avenue
>> New York, NY 10031
>> Tel. (212) 650-6070
>>
>>
>> ---- Original message
>> >Date: Fr
2014 13:57
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] ITC with unfolded proteins
That is a very interesting question, which I would request the seniors out
there to give their insights on.
I was imagining that a recombinant purification of an unfolded partner would
aggregate which would
t Professor
> The City College of New York
> Department of Chemistry, MR-1135
> 160 Convent Avenue
> New York, NY 10031
> Tel. (212) 650-6070
>
>
> Original message
> >Date: Fri, 14 Mar 2014 18:07:48 +0530
> >From: CCP4 bulletin board (on behalf
&g
10031
Tel. (212) 650-6070
Original message
>Date: Fri, 14 Mar 2014 18:07:48 +0530
>From: CCP4 bulletin board (on behalf
of Anita P )
>Subject: [ccp4bb] ITC with unfolded proteins
>To: CCP4BB@JISCMAIL.AC.UK
>
> Hello everyone,
> I have a query for the
Hello everyone,
I have a query for the scientists working on protein-protein interaction.
It is known that some proteins exist in unfolded or molten globule state
and attain structure on interaction with other folded proteins.
Many a times, it is difficult to obtain the structure of these complexe
