If the following is not deleterious to your protein and its function you
could introduce mutations that prevent dimerization.
~Jeff
> I have a similar case, where in there are multiple binding sites on the
> protein for the ligand and ligand induces dimerization.So it is not
> helpful
> even if I
n board [CCP4BB@JISCMAIL.AC.UK] de la part de Ramesh V
[ramesh.c...@gmail.com]
Envoyé : vendredi 18 juillet 2014 14:42
À : CCP4BB@JISCMAIL.AC.UK
Objet : Re: [ccp4bb] ITC with heterogeneous protein
I have a similar case, where in there are multiple binding sites on the protein
for the ligand and
I have a similar case, where in there are multiple binding sites on the
protein for the ligand and ligand induces dimerization.So it is not helpful
even if I separate the monomer and dimer.
If I titrate the dimer with ligand, the stoichiometry will completely
change? Any suggestions will be helpful
Hi Sajid,
*Assuming* you have one site per monomer (rather than, say, one site per
dimer), and *assuming* each binding event is completely independent ( I.e
no co-operativity), you might just get away with running the experiment
with the heterogeneous material.
However, you might not be able to c
-Message d'origine-
De : CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] De la part de sajid
akthar
Envoyé : vendredi 18 juillet 2014 11:24
À : CCP4BB@JISCMAIL.AC.UK
Objet : [ccp4bb] ITC with heterogeneous protein
Dear All,
This is an off-topic question. I have protein solution of heteroge
id akthar
[b_sajid_...@yahoo.co.in]
Envoyé : vendredi 18 juillet 2014 11:24
À : CCP4BB@JISCMAIL.AC.UK
Objet : [ccp4bb] ITC with heterogeneous protein
Dear All,
This is an off-topic question. I have protein solution of heterogeneous
(contains both monomer and dimer). I want to perform ITC with th
Dear All,
This is an off-topic question. I have protein solution of heterogeneous
(contains both monomer and dimer). I want to perform ITC with this protein. I
doubt whether this heterogeneity will interfere the binding study.
Any advice please.
Thank you
Sajid
