ober 4, 2019 5:06:10 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] ITC question -dimer vs monomer
Thanks to All for the extended & informative responses. If true thermodynamic
equilibria
are realized, then I would agree that regardless of the pathway the endpoint
(or integrated H)
sho
m. I may keep bugging selected victims off-board once I
have the first data.
Many thanks again, BR
From: CCP4 bulletin board On Behalf Of Barone,
Matthias
Sent: Thursday, October 3, 2019 18:59
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] ITC question -dimer vs monomer
As Reza alr
"Bernhard Rupp"
À: CCP4BB@JISCMAIL.AC.UK
Envoyé: Jeudi 3 Octobre 2019 17:05:56
Objet: [ccp4bb] ITC question -dimer vs monomer
Hi Fellows,
please let me ask the respective experts an ITC question: I have 2 proteins,
stable and dialyzed in identical buffer.
A is a monomer and B an obli
93-284
From: CCP4 bulletin board on behalf of Roger Rowlett
Sent: Thursday, October 3, 2019 8:36:27 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] ITC question -dimer vs monomer
Won't this depend on the relative final concentrations of A and B in the two
experiments? If A
nt:* Thursday, October 3, 2019 17:41
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* Re: [ccp4bb] ITC question -dimer vs monomer
>
>
>
> I don’t understand what you are trying to do—are you trying to show, by
> the difference in ITC response, that th
so try with SPR. Always best to do these things
> using multiple complimentary methods
>
>
>
> Best wishes
>
>
>
> Clare
>
>
>
> *From:* CCP4 bulletin board *On Behalf Of *Bernhard
> Rupp
> *Sent:* 03 October 2019 16:06
> *To:* CCP4BB@JISCMAIL.AC.UK
mp;source=web&rct=j&url=http://www.chem.gla.ac.uk/staff/alanc/itcdil.pdf&ved=2ahUKEwiOpoeisYDlAhUDThUIHX5mAyMQFjAAegQIAhAB&usg=AOvVaw0Ng-W03upy4DG12unFGDY3
From: CCP4 bulletin board On Behalf Of Bernhard Rupp
Sent: 03 October 2019 16:52
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4
2019 16:06
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] ITC question -dimer vs monomer
Hi Fellows,
please let me ask the respective experts an ITC question: I have 2 proteins,
stable and dialyzed in identical buffer.
A is a monomer and B an obligate dimer. I suspect that eventually a A2B2 dimer
em.gla.ac.uk/staff/alanc/itcdil.pdf&ved=2ahUKEwiOpoeisYDlAhUDThUIHX5mAyMQFjAAegQIAhAB&usg=AOvVaw0Ng-W03upy4DG12unFGDY3
From: CCP4 bulletin board On Behalf Of Bernhard Rupp
Sent: 03 October 2019 16:52
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] ITC question -dimer vs monomer
I am not
College of New York
Department of Chemistry
New York, NY 10031
From: CCP4 bulletin board on behalf of Bernhard Rupp
Sent: Thursday, October 3, 2019 11:51 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [EXTERNAL] Re: [ccp4bb] ITC question -dimer vs monomer
I am not looki
: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] ITC question -dimer vs monomer
I don't understand what you are trying to do-are you trying to show, by the
difference in ITC response, that the predictions you made about the
oligomerization are true?
Sent: Thursday, October 3, 2019 11:06 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] ITC question -dimer vs monomer
Hi Fellows,
please let me ask the respective experts an ITC question: I have 2 proteins,
stable and dialyzed in identical buffer.
A is a monomer and B an obligate dimer. I suspect
Hi Fellows,
please let me ask the respective experts an ITC question: I have 2 proteins,
stable and dialyzed in identical buffer.
A is a monomer and B an obligate dimer. I suspect that eventually a A2B2
dimer will form.
Intuitively, it should make a difference whether I titrate the dimer w
I recall a case of this a few years ago where it had to do with the
relative concentration of the protein to the buffer/salt molecules (can't
remember which anymore), which ended up being important in the binding that
was observed. So it was entirely a concentration effect that caused the
differenc
Le Vendredi 17 Mars 2017 16:07 CET, Nicholas Larsen
a écrit:
Do you mean that the affinity from ITC is 100-fold weaker ? Which would mean
that the Kd values from ITC are 100-fold larger (in the range 0.1-1 µM) ?
If this is the case, it makes me think to a problem that we have observed by
comp
Dear colleagues,
We have a target where people have measured Kd's for ligands using
radioligand binding assays. Several publications report Kd's of single
digit nanomolar and we are able to reproduce that data using this assay
format. When we try to do the same measurement using ITC, we generate
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