.
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Xuan
Yang
Sent: Wednesday, August 04, 2010 9:46 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] I-TASSER predicts NADPH binding, need to confirm with
experiment
Dear Jacob,
Nanodrop
concentrators, you can achieve the results that Jacob described.
Hope that helps,
Bryan
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Xuan Yang
Sent: Wednesday, August 04, 2010 9:46 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] I-TASSER predicts NADPH binding, need to
Dear Jürgen,
Thanks for the feedback.
Differential Scanning Fluorimetry is a nice method but my current goal is to
identify the nature of the cofactor if any. I was wondering whether MassSpec
could be of help.
Sincerely,
Xuan
2010/8/4 Jürgen Bosch
> Check the thermal stability with and witho
Dear Jacob,
Nanodrop ultrafiltration sounds really fancy to me. I am afraid that I have
no access to such equipment yet.
Thanks for letting me know about this new technology.
Sincerely,
Xuan Yang
2010/8/4 Jacob Keller
> I like nanodrop ultrafiltration:
>
> concentrate your protein to the highe
I like nanodrop ultrafiltration:
concentrate your protein to the highest stable concentration possible
figure out what is the lowest possible robustly-detectable nadph signal on
your nanodrop
combine the two in such a way in the top of a microcon of appropriate MWCO
to acheive the highest possib
Check the thermal stability with and without your ligand. You could do this via
CD or with the help of Sypro Orange in a RT-PCR machine.
Jürgen
..
Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Department of Biochemistry & Molecular Biology
Johns Hopkins Malaria
Dear All,
3D structure modeling server I-TASSER predicts a binding site for NADPH and
I want to test this prediction. What would be the nice quick way to tell
whether this protein bind NADPH or not, when I have a lot of recombinant
protein?
Sincerely,
Xuan Yang
