Hi Debajyoti,
Migration of proteins in SDS containing gels is dependent on hydrophobicity,
the amount of SDS that your protein binds (despite the fact that in theory
all proteins should behave the same way under these conditions) and charge.
Highly basic or acidic proteins will migrate anomalously
Dear All,
Sorry for the off topic question.
I am purifying one protein which is showing increased molecular weight +5kDa
(more than adding up the Hexa His and cloning artifact) in normal 12% SDS PAGE.
The DNA sequence is as it is. The gel runs without blurring the lanes and
without any difficu
