Meg
Sent: Friday, September 26, 2008 4:05 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] HydrophobiC interaction Chromo
Dear all,
In continuation with my earlier mail on problem in cation exchange chromato,
i
was suggested to go for hydrobic interaction chromato [HIC] as an additional
step. my p
Hi Meg,
ethanol is often used in a HIC elution buffer as it is good at
breaking hydrophobic/hydrophoic interactions. (Detergents or
isopropanol can be used to do the same thing). But, as with all
things, tolerance to ethanol depends upon your protein.
At pH 5, I would guess that Citrate or Acetat
Dear all,
In continuation with my earlier mail on problem in cation exchange chromato, i
was suggested to go for hydrobic interaction chromato [HIC] as an additional
step. my protein is indeed a hydrophobic protein. we have sepharose 6 fast
flow low sub resin and my protein has a pi of 5.8 -6.3
