Re: [ccp4bb] How to reduce no. of overlaps

2012-03-07 Thread Edward A. Berry
Jim Pflugrath wrote: In addition to reducing the beam divergence, you may wish to use a smaller beam size by using a smaller collimator or making the slits smaller. A smaller crystal can also help to spatially separate the Bragg spots as can moving the detector closer to the crystal. Yes, closer

Re: [ccp4bb] How to reduce no. of overlaps

2012-03-07 Thread Bosch, Juergen
In addition to all the excellent suggestions if you can you can also move your detector away from the center of the beam aka increase the detector size in one dimension. Not sure if you can do that at your home source though. By moving the center of the beam say to the lower 9/10 of the detector

Re: [ccp4bb] How to reduce no. of overlaps

2012-03-07 Thread Mark J van Raaij
my experiences: C2 with a long axis parallel to the shortest crystal dimension, crystals were plates. Used prebent loops to fish the crystals. Personally I haven't tried to bend loops in mounted crystals as Frank does, but it sounds very useful. bar-shaped P321 crystals with hexagonal cross-secti

Re: [ccp4bb] How to reduce no. of overlaps

2012-03-07 Thread Zhijie Li
@JISCMAIL.AC.UK Subject: [ccp4bb] How to reduce no. of overlaps Dear Crystallographers, I am working on a protein having SG P6, the cell parameters are a= 79, b= 79, c= 325. The crystals are forming in big size and with very good shape. It also diffracting very well in Home source facility

Re: [ccp4bb] How to reduce no. of overlaps

2012-03-07 Thread Frank von Delft
om/product_detail.aspx?cid=24&sid=136&pid=385> *From:* Frank von Delft <mailto:frank.vonde...@sgc.ox.ac.uk> *Sent:* Wednesday, March 07, 2012 1:26 AM *To:* CCP4BB@JISCMAIL.AC.UK <mailto:CCP4BB@JISCMAIL.AC.UK> *Subject:* Re: [ccp4bb] How to reduce no. of overlaps You prob

Re: [ccp4bb] How to reduce no. of overlaps

2012-03-07 Thread Jim Pflugrath
In addition to reducing the beam divergence, you may wish to use a smaller beam size by using a smaller collimator or making the slits smaller. A smaller crystal can also help to spatially separate the Bragg spots as can moving the detector closer to the crystal. Yes, closer to the crystal. Th

Re: [ccp4bb] How to reduce no. of overlaps

2012-03-06 Thread Zhijie Li
Hampton sells an adjustable mounted loop for this purpose. http://hamptonresearch.com/product_detail.aspx?cid=24&sid=136&pid=385 From: Frank von Delft Sent: Wednesday, March 07, 2012 1:26 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] How to reduce no. of overlaps You probably

Re: [ccp4bb] How to reduce no. of overlaps

2012-03-06 Thread Frank von Delft
Yes... quite. Thanks! (Beam, rotation axis... how can you expect me to keep all these new-fangled inventions apart?!) On 07/03/2012 07:33, VAN RAAIJ , MARK JOHAN wrote: typo correction, you'll want the long axis parallel to the rotation axis, not to the beam. Mark Quoting Frank von Delft:

Re: [ccp4bb] How to reduce no. of overlaps

2012-03-06 Thread VAN RAAIJ , MARK JOHAN
typo correction, you'll want the long axis parallel to the rotation axis, not to the beam. Mark Quoting Frank von Delft: You probably have to tilt your crystal, so that the long axis is parallel to the beam. We do this routinely: cut a plastic pipette tip to have a sharp point, then push

Re: [ccp4bb] How to reduce no. of overlaps

2012-03-06 Thread Frank von Delft
You probably have to tilt your crystal, so that the long axis is parallel to the beam. We do this routinely: cut a plastic pipette tip to have a sharp point, then push the loop where it attaches to the pin, to bend the crystal itself. You have to identify from your diffraction whether the lo

[ccp4bb] How to reduce no. of overlaps

2012-03-06 Thread Dipankar Manna
Dear Crystallographers, I am working on a protein having SG P6, the cell parameters are a= 79, b= 79, c= 325. The crystals are forming in big size and with very good shape. It also diffracting very well in Home source facility both in terms of resolution and intensity. But the only problem is t