Hi, Phil,
Sorry for the confusion. I did use MR solution/data combination to run
Refmac and I did reindex the data and re-run MR with the reindexed MTZ file
and run refmac. Both gave the above given high Rwork/Rfree values
(0.35/0.41). If I refine the original MTZ native data file with the PDB
Haiying,
As far as I can tell you've got a successful solution in molecular
replacement via Phaser and then gone and refined it in the wrong space
group.
Based on what you've told us: you took your initial data in primitive
orthorhombic and solved for the structure in Phaser while sampling
Hi, all
I have encountered the high Rwork/Rfree values. Here is the story:
The glycosylated native protein structure is solved in the P212121
with unit cell parameters of 72.6, 78.0, and 112.5 and the all
solution statistics are perfectly fine. I am trying to crystallize and
solve the structure o
Hi, all
I have encountered the high Rwork/Rfree values. Here is the story:
The glycosylated native protein structure is solved in the P212121 with unit
cell parameters of 72.6, 78.0, and 112.5 and the all solution statistics are
perfectly fine. I am trying to crystallize and solve the structure
