Re: [ccp4bb] High R/Rfree after MR

2017-10-16 Thread Diana Tomchick
Paul Steven Miller (PhD) > >> Postdoctoral Researcher > >> University of Oxford > >> Wellcome Trust Centre for Human Genetics > >> Division of Structural Biology > >> Roosevelt Drive > >> Oxford > >> OX3 7BN > >> > >&

Re: [ccp4bb] High R/Rfree after MR

2017-10-14 Thread Eleanor Dodson
iso whether anisotropy can > cause this problem and whether there is any way around it. > > >> > > >> Cheers, Paul > > >> > > >> Paul Steven Miller (PhD) > > >> Postdoctoral Researcher > > >> University of Oxford > > &g

Re: [ccp4bb] High R/Rfree after MR

2017-10-13 Thread Gerard Bricogne
und it. > >> > >> Cheers, Paul > >> > >> Paul Steven Miller (PhD) > >> Postdoctoral Researcher > >> University of Oxford > >> Wellcome Trust Centre for Human Genetics > >> Division of Structural Biology > >> R

Re: [ccp4bb] High R/Rfree after MR

2017-10-13 Thread Gianluca Cioci
; >> Postdoctoral Researcher > >> University of Oxford > >> Wellcome Trust Centre for Human Genetics > >> Division of Structural Biology > >> Roosevelt Drive > >> Oxford > >> OX3 7BN > >> > >> > >> Original mes

Re: [ccp4bb] High R/Rfree after MR

2017-10-13 Thread Randy Read
ty of Oxford >> Wellcome Trust Centre for Human Genetics >> Division of Structural Biology >> Roosevelt Drive >> Oxford >> OX3 7BN >> >> >> Original message >> >>> Date: Fri, 13 Oct 2017 10:30:22 +0200 >>> From: CCP4

[ccp4bb] AW: [ccp4bb] High R/Rfree after MR

2017-10-13 Thread Herman . Schreuder
Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Gianluca Cioci Gesendet: Freitag, 13. Oktober 2017 10:30 An: CCP4BB@JISCMAIL.AC.UK Betreff: [EXTERNAL] [ccp4bb] High R/Rfree after MR Dear All, I am trying to refine a structure at 3.3A. Model has 60% identity to the target

Re: [ccp4bb] High R/Rfree after MR

2017-10-13 Thread vincent Chaptal
al message Date: Fri, 13 Oct 2017 10:30:22 +0200 From: CCP4 bulletin board (on behalf of Gianluca Cioci ) Subject: [ccp4bb] High R/Rfree after MR To: CCP4BB@JISCMAIL.AC.UK Dear All, I am trying to refine a structure at 3.3A. Model has 60% identity to the target. Maps look OK (for 3.

Re: [ccp4bb] High R/Rfree after MR

2017-10-13 Thread Kay Diederichs
n Miller (PhD) >Postdoctoral Researcher >University of Oxford >Wellcome Trust Centre for Human Genetics >Division of Structural Biology >Roosevelt Drive >Oxford >OX3 7BN > > > Original message >>Date: Fri, 13 Oct 2017 10:30:22 +0200 >>From: CCP4 bulleti

Re: [ccp4bb] High R/Rfree after MR

2017-10-13 Thread Paul Miller
University of Oxford Wellcome Trust Centre for Human Genetics Division of Structural Biology Roosevelt Drive Oxford OX3 7BN Original message >Date: Fri, 13 Oct 2017 10:30:22 +0200 >From: CCP4 bulletin board (on behalf of Gianluca Cioci >) >Subject: [ccp4bb] High R/Rfree after MR

[ccp4bb] High R/Rfree after MR

2017-10-13 Thread Gianluca Cioci
Dear All, I am trying to refine a structure at 3.3A. Model has 60% identity to the target. Maps look OK (for 3.3A) and rebuilding in Coot is relatively straightforward. However, after some rebuilding cycles the R factors are stuck at 0.37/0.39 (REFMAC). XTRIAGE tells me that everything is normal (