-
> *From:* CCP4 bulletin board on behalf of Jonathan
> Bailey
> *Sent:* 31 July 2017 22:34
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* [ccp4bb] Help needed finding hit condition
>
>
> Dear CCP4bb community
>
>
> I apologies for the slightly off topic pos
han Bailey
Sent: 31 July 2017 22:34
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Help needed finding hit condition
Dear CCP4bb community
I apologies for the slightly off topic post.
We have recently had success crystallizing a membrane protein (diffraction > 3
Å at a synchrotron source) usin
Hi Jonathan,
Old buffer may have slow evaporation with some side reactions. The
concentration of each component may increase a little bit. In this case,
I would consider the condition as the origin for further optimization.
Each component may need to be considered separately.
For 150mM Tris pH
Jonathan,
While your claim of oxidative degradation of PEG1000 may be true -- I gather
you mean that the conversion of the ends of the PEG polymers to aldehydes or
peroxides, then to carboxylates -- you should check out Fran Jurnak’s old
paper (Journal of Crystal Growth 76, 577, 1986). The sy
buffering agent to… MES
perhaps ?
Cheers,
Fred.
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jonathan
Bailey
Sent: Monday, July 31, 2017 2:34 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Help needed finding hit condition
Dear CCP4bb community
I apologies for the slightly
Dear CCP4bb community
I apologies for the slightly off topic post.
We have recently had success crystallizing a membrane protein (diffraction
> 3 Å at a synchrotron source) using the *in meso* method, the hit
condition was from the Jena Bioscience screen Pi-minimal condition number
#57.
Hit c
