Re: [ccp4bb] Helix forming proteins crystallizing as rings

2013-06-05 Thread Bosch, Juergen
Hi Joern, have a look at these two papers (and probably KK is reading this too and can chime in with the right set of coordinates), Korotkov et al. Secretins: dynamic channels for protein transport across membranes. Trends Biochem Sci (2011) vol. 36 (8) pp. 433-43 Reichow et al. Structure of

Re: [ccp4bb] Helix forming proteins crystallizing as rings

2013-06-05 Thread Joern Krausze
Dear Tim, the rings I mean are similar to the one in 4FC4 but to my understanding FocA does not form helices under physiological conditions. I am sorry if I was not specific enough. I will give you more details. I am working on two proteins which are dimers in solution. The first of them crysta

Re: [ccp4bb] Helix forming proteins crystallizing as rings

2013-06-05 Thread Colin Nave
2013 11:38 To: ccp4bb Subject: Re: [ccp4bb] Helix forming proteins crystallizing as rings -BEGIN PGP SIGNED MESSAGE- Hash: SHA1 Dear Joern, I am not sure this is what you have in mind: just yesterday I heard a talk from Oliver Einsle about FocA ion channels - look at PDB ID 4FC4, for

Re: [ccp4bb] Helix forming proteins crystallizing as rings

2013-06-05 Thread Tim Gruene
-BEGIN PGP SIGNED MESSAGE- Hash: SHA1 Dear Joern, I am not sure this is what you have in mind: just yesterday I heard a talk from Oliver Einsle about FocA ion channels - look at PDB ID 4FC4, for example forming a (flat) pentamer, and let us know if this is what you mean. Best, Tim On 06

[ccp4bb] Helix forming proteins crystallizing as rings

2013-06-04 Thread Joern Krausze
Dear all, can anyone provide me with examples for proteins that form a helical oligomer under physiological conditions and flat rings (basically a helix with a pitch of 0) in the crystal? I only know examples of the other way around. Thank you in advance. Joern