Re: [ccp4bb] Heavy atom soaking

2008-02-15 Thread Tim Gruene
Could you try a quick (10-15s) halide soak (Iodine) with e.g. 1M NaI? Tim -- Tim Gruene Institut fuer anorganische Chemie Tammannstr. 4 D-37077 Goettingen GPG Key ID = A46BEE1A On Fri, 15 Feb 2008, john peter wrote: Dear Folks, I recently grew crystals in the presence of 100 mM Imidazole pH

[ccp4bb] Heavy atom soaking

2008-02-15 Thread john peter
Dear Folks, I recently grew crystals in the presence of 100 mM Imidazole pH 8.0 and 1 M Ammonium Phosphate. The protein was in 25 mM Hepes pH 7.4, 10 mM EDTA, 10 mM EGTA and 100 mM NaCl. I like to make heavy atom derivatives with Hg, Pt, Sm, Au and Pb compounds. I think the imidazole will inter