Could you try a quick (10-15s) halide soak (Iodine) with e.g. 1M NaI?
Tim
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Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
On Fri, 15 Feb 2008, john peter wrote:
Dear Folks,
I recently grew crystals in the presence of 100 mM Imidazole pH
Dear Folks,
I recently grew crystals in the presence of 100 mM Imidazole pH 8.0 and 1
M Ammonium Phosphate. The protein was in 25 mM Hepes pH 7.4, 10 mM EDTA, 10
mM EGTA and 100 mM NaCl. I like to make heavy atom derivatives with Hg, Pt,
Sm, Au and Pb compounds. I think the imidazole will inter