Rhys,
If crystals grow reproducibly and within a reasonable timeframe, I would always
do co-crystallization, particularly if the HA is a good anomalous scatterer.
We have had good success with this method, including a recent membrane protein
structure. Even if you get crystals that are not is
Rhys,
since you can obtain good native crystals, I would try Xenon phasing. I have
recently heard several success stories at the Australian Synchrotron, and it
just seems to be a straightforward and rational way.
Molecular Replacement should also be an option. I did this with beta-barrel
prote
Dear Rhys,
the humidity control device at Diamond and ESRF (HC1) is apparently
especially recommended for crystals that show large variability. You
might want to give this a try, collect a few wedges at RT off many
crystals and then Blend them. This approach might increase resolution
and, a
Hi Rhys,
Have you tried quick back-soaks into solutions lacking the heavy atoms? This
can reduce radiation decay caused by absorption of X-rays by overabundant heavy
atoms.
Best,
Chris
> On Jul 27, 2014, at 4:48 PM, RHYS GRINTER
> wrote:
>
> Hi All,
>
> I thought I might put a question t
anan
Fax: 972-8-647-2992 or 972-8-646-1710
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of RHYS GRINTER
[r.grinte...@research.gla.ac.uk]
Sent: Monday, July 28, 2014 12:48 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Heavy Atom Phas
8-647-2992 or 972-8-646-1710
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of RHYS GRINTER
[r.grinte...@research.gla.ac.uk]
Sent: Monday, July 28, 2014 12:48 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Heavy Atom Phasing
Hi All,
I thoug
Hi All,
I thought I might put a question to the community, with the hope of getting
some tips of the best way to proceed with my heavy atom phasing problem.
I'm working on solving the structure of an integral beta-barrel membrane
protein of approximately 100 kDa. I've crystallised protein, growi
