Re: [ccp4bb] Glutathione rsin

2013-06-05 Thread Katherine Sippel
Thermo Scientific/Pierce has gotten into the protein purification game recently. The resin is slightly cheaper (USD $975/100 ml versus $1656 for GE) and the binding capacity is equivalent. I haven't rigorously run any regeneration tests though. Our local sales rep actually sent us a 1 ml spin colum

Re: [ccp4bb] Glutathione rsin

2013-06-05 Thread Barbara Giabbai
Hi all. I don't have any experience with Clontech and Fisher resins, but about the GE one I faced the same problem as Sebastiano indicated. But I have to say that the problem was not general, but protein (or family of protein) related: the low binding depends on the oligomerization state of t

Re: [ccp4bb] Glutathione rsin

2013-06-05 Thread Sebastiano Pasqualato
Hi Mirek, hi all, I'm also very interested in the topic, so please keep me up with the replies, or make sure to post a summary, please. In addition to the price, the problem we're facing with GSH-beads from GE (although we haven't tried others yet) is that we can't manage to deplete our lysate

[ccp4bb] Glutathione rsin

2013-06-04 Thread Cygler, Miroslaw
Hi, I would like to ask the bb faithful for their experience with the glutathione affinity resins. We have been using so far the Glutathione Sepharose fast flow from GE but the price is getting steeper. We found Glutathione Superflow resin from Clontech to be significantly less expensive and Glu