Re: [ccp4bb] GFP membrane protein expression

2012-05-30 Thread David Drew
> Dear all > > Is there any method to check membrane protein overexpression using GFP when > the C terminus is in periplasm? My reading so far all mention that for C > terminus fusion to work, it has to be cytoplasm. > > Thank you. Dear Theresa, Superfolder GFP (sGFP) is reported to transloc

[ccp4bb] GFP membrane protein expression

2012-05-30 Thread Theresa Hsu
Dear all Is there any method to check membrane protein overexpression using GFP when the C terminus is in periplasm? My reading so far all mention that for C terminus fusion to work, it has to be cytoplasm. Thank you.