Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation

2014-12-16 Thread Patrick Shaw Stewart
ompression fit. > >> Maybe if you have a jig that holds the 2 syringes in a fixed position so > >> they cannot move apart it can work. > >> > >> Best, BR > >> > >> -Original Message- > >> From: CCP4 bulletin board [mailto:CCP4

Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation

2014-12-15 Thread Bernhard Rupp
, 2014 8:48 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation Here's my addition to Jim Fairman's reply: You could use a pair of RN compression fittings (www.hamilton dot com part number 55751-01) and a segment of HPLC tubin

Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation

2014-12-15 Thread Thomas Cleveland
jig that holds the 2 syringes in a fixed position so >> they cannot move apart it can work. >> >> Best, BR >> >> -Original Message- >> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of >> Daniel Anderson >> Sent: Monday, December 1

Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation

2014-12-15 Thread Aaron Thompson
t; they cannot move apart it can work. > > Best, BR > > -Original Message- > From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of > Daniel Anderson > Sent: Monday, December 15, 2014 8:48 PM > To: CCP4BB@JISCMAIL.AC.UK > Subject: Re: [ccp4bb] Fabric

Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation

2014-12-15 Thread Bernhard Rupp
apart it can work. Best, BR -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Daniel Anderson Sent: Monday, December 15, 2014 8:48 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation Here'

Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation

2014-12-15 Thread Daniel Anderson
correction: www.hamiltoncompany.com addition: The compression fittings at least used to be available from us.vwr.com part number 89187-0002. On 12/15/2014 11:48 AM, Daniel Anderson wrote: Here's my addition to Jim Fairman's reply: You could use a pair of RN compression fittings (www.hamilton

Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation

2014-12-15 Thread Jarrod Mousa
Hi Thomas, I have been doing LCP for a while and I am using the teflon ferrules that come with the 250 uL RN syringes instead. So on one side you would have two teflon ferrules and the other side you would have the ferrule that is attached to the needle and another plastic ferrule. I have also had

Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation

2014-12-15 Thread Daniel Anderson
Here's my addition to Jim Fairman's reply: You could use a pair of RN compression fittings (www.hamilton dot com part number 55751-01) and a segment of HPLC tubing. HPLC tubing within my field of view can have an inside diameter as small as 0.005 inch. hope that helps, Happy Merry, etc., Da

Re: [ccp4bb] Fabricating hamilton syringe coupler for LCP preparation

2014-12-15 Thread Jim Fairman
As a user of many of these coupling devices, I would highly suggest buying your couplers pre-fabricated and avoiding many hours (and possibly days) of headaches trying to manufacture these with your own hands. Rigaku Reagents sells them: https://www.rigakureagents.com/p-516-wizard-cubic-lcp-kit.as

[ccp4bb] Fabricating hamilton syringe coupler for LCP preparation

2014-12-15 Thread Thomas Cleveland
Hi all, I'm trying to put together some homemade syringe couplers following the published instructions from the Caffrey group. I'm having a bit of trouble with this part: "The stainless steel ferrule of the second needle is removed and placed on the free end of the coupling needle such that the