Hi Rafael,
If it has not been already suggested: try DMSO (20% to 40%).
In my limited experience I found that often DMSO works well for
crystallization conditions with high-salt or high buffer component
(like >1M D,L,-Malic acid).
HTH,
-Partha
On Thu, Dec 17, 2009 at 1:39 PM, Meitian Wang wrot
good point! recently we managed to collect very good room temperature
data with PILATUS detector at SLS. if your crystals are large enough,
say 100 microns or so, you have chance. regards, meitian
On Dec 15, 2009, at 1:42 PM, mjvanraaij wrote:
why not stay with room temp?
many structure
Hi Rafael,
If you really want to diffract your crystals frozen, I have two more
suggestions for cryo-procedures which can be tried:
a) annealing
e.g.
Harp, J.; Timm, D. & Bunick, G. Macromolecular crystal annealing:
overcoming increased mosaicity associated with cryocrystallography.
Acta Crystallo
: 773.608.9185
email: j-kell...@northwestern.edu
***
- Original Message -
From: "Natalie Zhao"
To:
Sent: Tuesday, December 15, 2009 6:20 AM
Subject: [ccp4bb] FW: [ccp4]: TDS upon flashcooling
-Original Message-
From: owner-c..
With salt-based conditions sodium malonate is your friend:
Acta Cryst D59: 2356
On Tue, 2009-12-15 at 12:20 +, Natalie Zhao wrote:
> -Original Message-
> From: owner-c...@dl.ac.uk [mailto:owner-c...@dl.ac.uk] On Behalf Of Rafael
> Couñago
> Sent: 14 December 2009 20:22
> To: c...@ccp
You could try conditioning your crystals in a dehydration device (such
as the FMS (proteros) or the HC1 (EMBL)). Even if you crystals don't
improve from dehydration they can often be cryocooled without any
cryoprotectant after the mother liquor has been removed. We offer the
HC1b at the ESRF
Did you also try a cryo salt (e.g. Li+)? In the best case the xtals might even
grow in there.
GL
Jan
--- Natalie Zhao schrieb am Di, 15.12.2009:
Von: Natalie Zhao
Betreff: [ccp4bb] FW: [ccp4]: TDS upon flashcooling
An: CCP4BB@JISCMAIL.AC.UK
Datum: Dienstag, 15. Dezember 2009, 13:20
You can also set your cryostream to something like 253K... or even lower
with high salt. Better than RT and still no freezing. You need to use
one of these new loop covers to prevent drying out.
Flip
mjvanraaij wrote:
why not stay with room temp?
many structures have been solved at RT...
Ma
why not stay with room temp?
many structures have been solved at RT...
Mark J. van Raaij
Dpto de Bioquimica, Facultad de Farmacia
Universidad de Santiago
15782 Santiago de Compostela
Spain
http://web.usc.es/~vanraaij/
researcherID: B-3678-2009
On 15 Dec 2009, at 13:20, Natalie Zhao wrote:
-Original Message-
From: owner-c...@dl.ac.uk [mailto:owner-c...@dl.ac.uk] On Behalf Of Rafael
Couñago
Sent: 14 December 2009 20:22
To: c...@ccp4.ac.uk
Subject: [ccp4]: TDS upon flashcooling
Dear all,
I got these beautiful looking crystals that grow in high salt (1.8M) and
diffract under
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