Re: [ccp4bb] Extreme off-topic on protein expression

2016-11-12 Thread Artem Evdokimov
Impossible to say without knowing details of the particular protein. Could be trivial or could be impossible. Artem www.harkerbio.com "See us at PSDI 2016 in Malmo" (some messages are just what they seem) On Nov 13, 2016 1:04 AM, "Israel Sanchez" wrote: > Dear all, > > for reasons difficult to

Re: [ccp4bb] Extreme off-topic on protein expression

2016-11-12 Thread Keller, Jacob
, November 12, 2016 7:03 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Extreme off-topic on protein expression Dear all, for reasons difficult to explain in a brief email, I am interested in flipping the order of domains in a poli-peptide in bacteria. The scenario would be a multi-domain protein

[ccp4bb] Extreme off-topic on protein expression

2016-11-12 Thread Israel Sanchez
Dear all, for reasons difficult to explain in a brief email, I am interested in flipping the order of domains in a poli-peptide in bacteria. The scenario would be a multi-domain protein, let's say containing 3 domains DI, DII and DIII, which are arranged N-ter to C-ter: DI-DII-DIII. The protein