Thanks for all the replies {off list}.
Now it's wait and hope that this crystallization experiment is succesful.
Quite common. And it may be a good sign for crystallizability in those
conditions. As the concentration gradients dissipate from the initially
mixed drop, your protein re-solubilizes.
Roger Rowlett
On Aug 17, 2012 5:31 PM, "Yuri Pompeu" wrote:
> Hello everyone,
> I have just made a curious obser
Hello everyone,
I have just made a curious observation.
I purified an enzyme domain and concentrated it to around 16 mg/mL without much
mischief.
I then proceeded to set-up some drops. After 5 min most of the drops had a
muddy appearance to them which led me to think I was probably too concentrat
