Hi Dhiraj - In addition to the other good suggestions, you may also wish to
try codon optimization and different secretion signal peptides if your
target protein is secreted.
Hope that helps.
Cheers,
Jared
On Thu, Jun 24, 2021 at 4:42 PM Srivastava, Dhiraj <
dhiraj-srivast...@uiowa.edu> wrote:
From: CCP4 bulletin board on behalf of Srivastava,
Dhiraj
Sent: Thursday, June 24, 2021 9:42:32 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Eukaryotic protein expression
External Sender: Use caution.
Hi all
I am trying to express my protein in HEK293 cells for crystallization
pu
Hi Dhiraj,
pHLsec vector for transient and its variants for lentivirus-based
expression (PMIDs 17001101 and 30455477, respectively).
Best wishes,
Tomas
On Thu, Jun 24, 2021 at 9:42 PM Srivastava, Dhiraj
wrote:
>
> Hi all
> I am trying to express my protein in HEK293 cells for crystalliz
Hi all
I am trying to express my protein in HEK293 cells for crystallization
purpose but getting poor expression. I am using cmv promoter. Which
promoter/vector people use to get good expression in eukaryotic expression
system?
Thank you
Dhiraj
