Dear Tristan and all my ccp4bb members,
Thank you so much for your valuable suggestions into the enzyme kinetics
issue I have been facing. It gave me new insights to design my experiment
and I started working on those inputs. Thank you all once again. It's very
helpful.
With kind regards
Prem
O
Is your enzyme monomeric or multimeric?
What time exactly does it mean when you say unprocessed substrate versus
processed?
What are your error margins (no error bars evident on your plots)?
Depending on your errors, your second plot is not a 'steep decline' but
instead could be a constant line.
Hello, you could also try:
http://www.ic50.tk/kmvmax.html
Or
http://ic50.org/kmvmax.html
The former has an exponential decay thing that might help with your substrate
inhibition and other things if you have biphasic behaviour ;-0
Cheers, Jon.C.
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Original
t; Hope this helps,
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>
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> Nic
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> *From:* CCP4 bulletin board *On Behalf Of *Tristan
> Croll
> *Sent:* 18 June 2021 08:19
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* Re: [ccp4bb] Enzyme Vmax and Km
>
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> Hi Prem,
>
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> The immediat
CP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Enzyme Vmax and Km
Hi Prem,
The immediate problem here is that the curve for the processed substrate simply
cannot be described by simple Michaelis-Menten kinetics. Assuming the assay has
worked as expected, the declining rate with increasing substrate concentr
Hi Prem,
The immediate problem here is that the curve for the processed substrate simply
cannot be described by simple Michaelis-Menten kinetics. Assuming the assay has
worked as expected, the declining rate with increasing substrate concentration
suggests to me that this substrate also acts as
