> cryoprotectant. Mitegen sells plastic capillaries, which you can slide
>> > over your loop to prevent the crystal from drying out.
>> >
>> > Good luck!
>> > Herman
>> >
>> > -Original Message-
>> > From: CCP4 bulletin b
r loop to prevent the crystal from drying out.
>
> Good luck!
> Herman
>
> -Original Message-
> From: CCP4 bulletin board
> [mailto:CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>] On Behalf Of
> Yi-Liang Liu
> Sent: Thursday, August 02, 2012 4:15 A
AC.UK] On Behalf Of
> > Yi-Liang Liu
> > Sent: Thursday, August 02, 2012 4:15 AM
> > To: CCP4BB@JISCMAIL.AC.UK
> > Subject: Re: [ccp4bb] Enhancing Crystal Quality
> >
> > Hi,
> >
> > Thanks for the kindly answers from everyone. I actually haven'
er your loop to prevent the crystal from drying out.
>
> Good luck!
> Herman
>
> -Original Message-
> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
> Yi-Liang Liu
> Sent: Thursday, August 02, 2012 4:15 AM
> To: CCP4BB@JISCMAIL.A
: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Yi-Liang Liu
Sent: Thursday, August 02, 2012 4:15 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Enhancing Crystal Quality
Hi,
Thanks for the kindly answers from everyone. I actually haven't tried
different cryoprotectan
Hi,
Thanks for the kindly answers from everyone. I actually haven't tried different
cryoprotectants. I might will give a try next time. I usually only use mother
liquor+30% PEG400. It is noticeable that it has some "patterns (cracks (?))" on
the crystal. However, it didn't form icy rings or etc
You might be killing crystals with the cryosolvent. Try soaking the
crystals briefly in well solution + 25-30% glucose or sucrose prior to
flash cooling. You can also try other cryoprotectants: glycerol, EG, etc.
You can also try sequential brief soaks in gradually increasing
cryoprotectant concent
Have you tried different cryoprotectants? Can make a huge difference. Also,
have you shot an xtal at room temp - to see what the intrinsic diffraction
limit is? Additive screens? If all else fails you may well need to explore a
different expression construct.
Tony.
Sent from my iPhone
On 1
Hi CCP4BBers,
I have a protein crystallized with 0.2mM Mg acetate and PEG 8000 or 0.1mM
cacodylate, pH 6.5, 0.2 Mg acetate, PEG 3350. Both of the conditions gave
triangle pyramid like crystals. I brought the crystals to synchrotron using 30%
PEG 400 as cryoprotactant, the resolution was only be
