Re: [ccp4bb] AW: [EXTERNAL] Re: [ccp4bb] Electron density maps for Cryo-EM structures.

2018-10-04 Thread John Berrisford
mobile phone… John From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Zhijie Li Sent: 10 September 2018 20:42 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] AW: [EXTERNAL] Re: [ccp4bb] Electron density maps for Cryo-EM structures. Hi Kevin, a) If your goal is merely to

Re: [ccp4bb] AW: [EXTERNAL] Re: [ccp4bb] Electron density maps for Cryo-EM structures.

2018-09-10 Thread Zhijie Li
o:CCP4BB@JISCMAIL.AC.UK> *Betreff:* [EXTERNAL] Re: [ccp4bb] Electron density maps for Cryo-EM structures. Hi Marin I was about to comment on that too but then I realised that Pavel is referring to the map _contours_  (which is what most people using a map visualisation

Re: [ccp4bb] AW: [EXTERNAL] Re: [ccp4bb] Electron density maps for Cryo-EM structures.

2018-09-10 Thread Kevin Jin
ontour level as you like. >> >> >> >> Of course, if the authors did not deposit their EM-map, you cannot >> download it, but the same is true for X-ray structure factors. >> >> >> >> Happy viewing! >> >> >> >> Herman &g

Re: [ccp4bb] AW: [EXTERNAL] Re: [ccp4bb] Electron density maps for Cryo-EM structures.

2018-09-10 Thread Garib Murshudov
the authors did not deposit their EM-map, you cannot download > it, but the same is true for X-ray structure factors. > > > > Happy viewing! > > > > Herman > > > > > > > > Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK > <mailto:

Re: [ccp4bb] AW: [EXTERNAL] Re: [ccp4bb] Electron density maps for Cryo-EM structures.

2018-09-10 Thread Kevin Jin
structure factors. > > > > Happy viewing! > > > > Herman > > > > > > > > *Von:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *Im Auftrag von > *Ian Tickle > *Gesendet:* Montag, 10. September 2018 12:58 > *An:* CCP4BB@JISCMAIL.AC.UK > *Betreff:* [

Re: [ccp4bb] Electron density maps for Cryo-EM structures.

2018-09-10 Thread Natesh Ramanathan
Dear Kevin, Are you referring to "generating a map" from the model (PDB coordinates) generated from tracing the chain in the ab-initio EM map? Best wishes, Natesh On Mon, 10 Sep 2018 at 09:58, Kevin Jin wrote: > Dear All, > > Is there any sever available to create electron density

[ccp4bb] AW: [EXTERNAL] Re: [ccp4bb] Electron density maps for Cryo-EM structures.

2018-09-10 Thread Herman . Schreuder
download it, but the same is true for X-ray structure factors. Happy viewing! Herman Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Ian Tickle Gesendet: Montag, 10. September 2018 12:58 An: CCP4BB@JISCMAIL.AC.UK Betreff: [EXTERNAL] Re: [ccp4bb] Electron density maps for

[ccp4bb] AW: [ccp4bb] Electron density maps for Cryo-EM structures.

2018-09-10 Thread Hughes, Jon
] Electron density maps for Cryo-EM structures. Hi Marin I was about to comment on that too but then I realised that Pavel is referring to the map _contours_ (which is what most people using a map visualisation program like Coot actually see). So the contoured map does represent an iso

Re: [ccp4bb] Electron density maps for Cryo-EM structures.

2018-09-09 Thread Kevin Jin
Great, thanks! On Sun, Sep 9, 2018 at 9:49 PM Pavel Afonine wrote: > P.S.: all questions are welcome of course, no labeling. It's just some of > them are so orthogonal to common sense that answers my be such as well. > Best, > Pavel > > On Sun, Sep 9, 2018 at 9:38 PM Pavel Afonine wrote: > >>

Re: [ccp4bb] Electron density maps for Cryo-EM structures.

2018-09-09 Thread Pavel Afonine
P.S.: all questions are welcome of course, no labeling. It's just some of them are so orthogonal to common sense that answers my be such as well. Best, Pavel On Sun, Sep 9, 2018 at 9:38 PM Pavel Afonine wrote: > Hi, > > Is there any sever available to create electron density maps for cryo-em >>

Re: [ccp4bb] Electron density maps for Cryo-EM structures.

2018-09-09 Thread Pavel Afonine
Hi, Is there any sever available to create electron density maps for cryo-em > structures? > The questions are nonsensical. Here is why: 1) In cryo-EM maps are not electron density maps but surfaces representing electric potential. 2) Creating such a map is essentially carrying on from cryo-EM

[ccp4bb] Electron density maps for Cryo-EM structures.

2018-09-09 Thread Kevin Jin
Dear All, Is there any sever available to create electron density maps for cryo-em structures? Or, we should create the maps from mmCIF. I am particularly interested in those cryo-em structures with high resolution, like 2.6~2.8A. Please give me an education. Thanks, Kevin ###