Many thanks to all who took out some time to reply.
Summary:
you can also use
https://www.neb.com/en/products/c3037-nebexpress-iq-competent-e-coli-high-efficiency
this has LacIq, we use it with all our pQE bassed vectors.
Gentauer may still sell those…
https://maxanim.com/strains/m15-prep4-esch
Hi Mark,
Gentauer may still sell those…
https://maxanim.com/strains/m15-prep4-escherichia-coli-strains/
Cheers,
Jeroen
__
Dr. math. et dis. nat. Jeroen R. Mesters
Biological Safety Officer (BBS)
Deputy, Lecturer, Program Coordinator Infection Biology
Visiting Professorship in Biophysics South B
o: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] E. coli strain M15 or SG13009
Dear All.
we are looking for E. coli strain M15 or SG13009 to use for expressing a
protein from the vector pQE30. Are these strains still availabe commercially?
If not, we'd be open for another way to obtain an aliquot.
Wbw
Dear All.
we are looking for E. coli strain M15 or SG13009 to use for expressing a
protein from the vector pQE30. Are these strains still availabe commercially?
If not, we'd be open for another way to obtain an aliquot.
Wbw,
Mark
Mark van Raaij
Dpto de Estructura de Macromoleculas, lab 20B
Ce
