Dear Abris,
if you have a partial MolRep solution and SAD data you might try to
combine both with phaser.
There is an example script here how to combine the model and SAD data to
determine and refine the heavy atom substructure:
https://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php?t
Estimating isomorphism by eyeballing the difference in unit cell lengths may
mask significant non-isomorphism that is present. Iodine is a large atom and it
is notoriously difficult to get an iodine derivative that is isomorphous to a
native protein.
Depending upon the chemistry of your mother
Dear all,
I’m trying to solve the structure of a putative homodimer, but despite having
collected good quality data, it has proven to be rather difficult.
I have collected native and derivative datasets (2.2-4Å, C2 SG,
165/35/115Å//104° unit cell) with acceptable merging statistics. Xtriage,
A
