Hi,
If I were you, I would collect a redundant dataset (~15-20 or even higher if
possible) at home and use the anomalous flag in Scalepack/Denzo. You should
be able to pick up the anomalous differences (especially with data to 2.0A)
for Se, even at CuKa wavelength at home!
Good luck!
<>
some good phasing.
Tassos
From: CCP4 bulletin board [ccp...@jiscmail.ac.uk] On Behalf Of Narayanan
Ramasubbu [ramas...@umdnj.edu]
Sent: Thursday, December 10, 2009 4:40 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Difficult MR structures
Deal All:
I have
Dear Subbu,
One more thing you can do is to search with an ensemble of structures (4
in your case) for molecular replacement.
Fred.
Narayanan Ramasubbu wrote:
Deal All:
I have a 2.0 A data for a SeMet protein (native crystal not available
yet!) that has 6 Se sites.
The cell comes out to be
Deal All:
I have a 2.0 A data for a SeMet protein (native crystal not available
yet!) that has 6 Se sites.
The cell comes out to be 65 67 101 and the angles are all very close to
90. The data set was collected in house with Cu 1.5418 A
We integrated and scale in orthorhombic and the statistics
