The same goes for other DNA-binding dyes. I've seen a polymerase that binds
EtBr.
-Joel
On Tue, Jun 23, 2009 at 1:38 PM, Kevin Jude wrote:
> Note that, despite the claim otherwise in Kettenberger and Cramer, SYBR
> Gold does stain at least some proteins, so be sure to run the appropriate
>
Hi, Kevin,
Wash your crystal very very well to get rid of unbound DNA. Then run
your crystal with SDS PAGE gel, with protein and DNA as markers. After
staining the gel with silver stain, the DNA will show with different
color and migrate about where the dye locates. So don't run too long
time.
Reg
Note that, despite the claim otherwise in Kettenberger and Cramer, SYBR Gold
does stain at least some proteins, so be sure to run the appropriate
controls.
kmj
On Tue, Jun 23, 2009 at 11:52 AM, Allyn Schoeffler wrote:
> Dear Nick,
>
> If you have access to a fluorescent microscope, you can try
Dear Nick,
If you have access to a fluorescent microscope, you can try staining
crystals with PicoGreen and seeing if they fluoresce. (see ref:
Kettenberger H & Cramer P, 2006, Acta Cryst D v62 pp146-150:
Kettenberger H, Cramer P.Fluorescence detection of nucleic acids and
proteins in multi-compo
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***
- Original Message -
From: "Nick Quade"
To:
Sent: Tuesday, June 23, 2009 8:06 AM
Subject: [ccp4bb] Detection
Dear all,
I am trying to solve the structure of a transcription factor in complex
with its DNA. I got crystals of the complex under different conditions
than the protein alone and they also look different. Unfortunately, they
only diffract to 6Å so far. Before I continue to optimize the crystals
