Hi Everyone,
Just wanted to let everyone know that i was able to process this dataset
with XDS ( and lots help from experts !)
Thanks again
Mahesh
On Mon, Aug 19, 2013 at 5:07 PM, Petri Kursula wrote:
> I have often processed images like this with XDS. Of course, you will get
> a better qual
Thank you experts for your valuable suggestions. I think Ill try to solve
it by proper data collection strategy the next time as i am unable to
process my current data even with the tricks that were mentioned here.
Thanks again
Mahesh
On Fri, Aug 16, 2013 at 2:04 PM, Bosch, Juergen wrote:
> ti
tilted is what I meant at an angle of e.g. 30 or 60 degrees. Works fine with
most SSRL beamlines except of the 12-2 microfocus - but that might have been
fixed in the meantime.
Jürgen
On Aug 16, 2013, at 1:57 PM, Bosch, Juergen wrote:
for #2)
I'd suggest get some of those Mitigen loops that a
This is continuation of the previous message, which was sent prematurely.
In case of crystals with one, very long unit cell data collection strategy
needs to be carefully chosen.
Ad. 2) What would be a better way to collect data in the future?
First, the detector needs to be placed far back enou
This is clearly a case of a crystal with a very long unit cell; a case
which should be approached mindfully.
HKL2000 has a default search for indexing solutions such that diffraction
along the longest unit cell will be resolved, with the assumed spot size.
The problem with such diffraction has 2
for #2)
I'd suggest get some of those Mitigen loops that are titled. I assume you have
hexagonal plates as crystals and you really want to shoot along the thin area
of the crystal down the sixfold. With normal loops it's an art to get that
crystal to sit upright in the loop but not impossible i
This is clearly a case of a crystal with a very long unit cell; a case
which should be approached mindfully.
HKL2000 has a default search for indexing solutions such that diffraction
along the longest unit cell will be resolved, with the assumed spot size.
The problem with such diffraction has 2
Hi Mahesh
In addition to what Mark and Juergen have written, it looks to me like you have
very high mosaicity in one direction - the first image shows no distinct lunes;
I suspect that may have something to do with the failure to process - but what
do you mean when you say you "cannot process i
PS For future data collections, try to get the long c-axis roughly along the
crystal rotation axis if possible.
On 16 Aug 2013, at 16:38, Mahesh Lingaraju wrote:
> Hi CCP4 folks
>
> I have a data set which is looks twinned ( see the image-1 - I zoomed on to
> the image so that one can spot t
doesn't necessarily looked twinned to me.
Rather it looks like you have a trigonal or hexagonal cell with a long c-axis.
On image 2 it seems you may have systematic absences along l, although it is
hard to tell the order. Perhaps P31, P32, P62, P64 or spacegroups with these
symmetries plus 2-fold
Hi Mahesh,
TESTGEN START 1 END 120 ANGLE 0.5
moving the detector further back or using 2theta and collecting more frames.
When you started shooting at your image you should have checked for overlaps
ahead of time.
I assume this to about 1.8 Å - maybe it would have been wiser to collect a 2.5
Å
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